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KMID : 0359319930330030471
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Korean Journal of Veterinary Research
1993 Volume.33 No. 3 p.471 ~ p.478
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Experimental colonization of Culicoides arakawae in laboratory
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김기석/Kim, Ki Seok
최상호/박근식/위성환/주후돈/Choi, Sang Ho/Park, Keun Sik/Wee, Sung Hwan/Joo, Hoo Don
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Abstract
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Culicoides arakawae is a kind of the main blood sucking insects of domestic fowls and serves as a vector of Leukocytozoom caulleryi, the causative protozoon of chicken leukocytozoonosis.
In this study, the complete life history of C arakawae was cycled by laboratory colonization.
Adult midges were collected from various poultry farm by light trap. The laboratory colonization was performed under the conditions of constant temperature of 25±1℃ and relative humidity of 80% or above. The hatched larvae were cultured in larval medium consisted of rice field mud and activated charcoal powder. The surface of medium was continuously flowed with biologically conditioned water. The fine powder meal composed of pellet feed for mice and equal mount of yeast was supplied for feeding larvae at every 72 hours.
The life cycle completed at 25℃ in 35∼35 days ; the period of preoviposition, egg. larval and pupal stage was 2∼3, 3∼4, 28∼30 and 3 days, respectively. The measurements of the eggs, the lst instar larvae, the 4th instar larvae and pupae was 36.28㎛±1.95, 13.58㎛±0.72, 4000㎛±1.47 and 219.95㎛±6.25 in mean±S.D., respectively.
In order to confirm experimental colonization of C arakawae in laboratory, the colonized adult midges were allowed to suck blood from chicken infected with L caulleryi. The oocysts and sporozoites could be identified in midguts and salivary grands of engorged midges at 72 hours after blood sucking.
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